吴鹤云,工学博士,太阳成集团tyc7111cc副教授,硕士生导师。
科研领域及方向
代谢工程与合成生物学,主要专注于高附加值氨基酸、核苷及其衍生物菌株构建和发酵过程调控。
讲授课程
本科生:《发酵工程原理与实验》《代谢控制发酵》
科研项目情况
[1] 国家重点研发计划:工业菌株重编程优化及应用(2022YFA0911800),2023-2027,子课题负责人
[2] 国家自然科学基金青年项目:高浓度L-组氨酸对大肠杆菌的生理调控及其耐受机理(32200038),2023-2025,主持
[3] 国家自然科学基金面上项目:限铵条件下谷氨酸棒杆菌生产α-酮戊二酸机制的研究(31770053),2018-2021,参与
[4] 横向项目:组氨酸新菌种构建及新工艺开发,2023-2028,项目负责人
[5] 横向项目:生物发酵法生产麦角硫因技术,2022-2023,项目负责人
[6] 横向项目:生物发酵法生产N-乙酰神经氨酸技术,2023-2025,项目负责人
主要学术成果
发表的主要学术论文有:
[1] Wu H, Li Y, Ma Q, Li Q, Jia Z, Yang B, Xu Q, Fan X, Zhang C, Chen N, Xie, X. Metabolic engineering of Escherichia coli for high-yield uridine production.2018. Metabolic Engineering. (共同一作)
[2] Wu H, Tian D, Fan X, Fan W, Zhang Y, Jiang S, Wen C, Ma Q, Chen N, Xie X. Highly efficient production of L-histidine from glucose by metabolically engineered Escherichia coli.2020. ACS Synthetic Biology.(共同一作)
[3] Fan X, Wu H, Li G, Yuan H, Zhang H, Li Y, Xie X, Chen, N. Improvement of uridine production of Bacillus subtilis by atmospheric and room temperature plasma mutagenesis and high-throughput screening.2017. Plos One.(共同一作)
[4] Fan X, Wu H, Jia Z, Li G, Li Q, Chen N, Xie, X.Metabolic engineering of Bacillus subtilis for the co-production of uridine and acetoin. 2018. Applied Microbiology and Biotechnology.(共同一作)
[5] Ma Q, Xia L, Wu H, Zhuo M, Yang M, Zhang Y, Tian M, Zhao K, Sun Q, Xu Q, Chen N, Xie, X. Metabolic engineering of Escherichia coli for efficient osmotic stress-free production of compatible solute hydroxyectoine. 2022 Biotechnology and Bioengineering. (共同一作)
[6] Xiong B, Zhu Y, Tian D, Jiang S, Fan X, Ma Q, Wu H, Xie X. Flux redistribution of central carbon metabolism for efficient production of L-tryptophan in Escherichia coli. 2021. Biotechnology and Bioengineering. (共同通讯)
[7] Jiang S, Wang D, Wang R, Zhao C, Ma Q, Wu H, Xie X. Reconstructing a recycling and nonauxotroph biosynthetic pathway in Escherichia coli toward highly efficient production of L-citrulline. 2021. Metabolic Engineering. (共同通讯)
[8] Jiang S, Wang R, Wang D, Zhao C, Ma Q, Wu H, Xie X. Metabolic reprogramming and biosensor-assisted mutagenesis screening for high-level production of L-arginine in Escherichia coli. 2023. Metabolic Engineering. (共同通讯)
[9] Li Y, Yan F, Wu H, Li G, Han Y, Ma Q, Fan X, Zhang C, Xu X, Xie X, Chen, N. Multiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli. 2019. Journal of Industrial Microbiology and Biotechnology.
[10] 吴鹤云,张悦,蒋帅,田道光,谢希贤.木糖和葡萄糖共发酵生产L-组氨酸.2020.食品与发酵工业.
[11] 刘铁重,高志强,侯德欣,谢希贤,吴鹤云.大肠杆菌IMP节点代谢优化高效合成肌苷.2023.食品与发酵工业.(通讯作者)
申请和授权发明专利主要有:
[1] Xixian Xie, Ning Chen, Heyun Wu, Guoliang Li, Qing Li, Xiaoguang Fan, Qingyang XU, Chenglin Zhang, Yanjun Li, Qian Ma. Genetically engineered bacterium used for producing uridine with high-yield. US11124780.
[2] Xixian Xie, Heyun Wu, Jiachu Wang, Faqing Wu, Xiaoqian Liu, Yanan Hao. Genetically engineered strain with high yield of L-valine and method for producing L-valine by fermentation. PCT/CN2019/114288
[3] 谢希贤,陈宁,吴鹤云,徐庆阳,张成林,范晓光,袁辉.一株高产嘧啶核苷的菌株及其氨甲酰磷酸合成酶调节位点.ZL2015110351418
[4] 谢希贤,陈宁,吴鹤云,李国梁,李强,范晓光,徐庆阳,张成林,李燕军,马倩.高产尿苷的基因工程菌及其构建方法与应用.ZL2018100209443.
[5] 谢希贤,樊伟明,吴鹤云,蒋卫,田道光,陈燕娜,张悦,屠建情.一种生产L-组氨酸的基因工程菌及其应用.ZL202010151245X.
[6] 谢希贤,陈宁,吴鹤云,徐庆阳,张成林,范晓光,张红超.高产嘧啶核苷的菌株及其氨甲酰磷酸合成酶调节位点,ZL2015110350701.
[7] 吴鹤云,刘万才,谢希贤,蒋帅,马倩,帅珍龙.一种生产麦角硫因的基因工程菌及其构建方法与应用,2022115465415.
[8] 吴鹤云,伍法清,谢希贤,姚卓越,侯德欣.一种生产L-缬氨酸的基因工程菌及其构建方法与应用,2022117421303.
[9] 吴鹤云,郭金玺,谢希贤,蒋帅,高志强,帅珍龙.利用弱化了rhaB基因的大肠杆菌提高细胞内ATP水平的方法,2022116787967.
[10] 吴鹤云,李淼,谢希贤,蒋帅,沈冠同,邵伟峻.一种生产L-丝氨酸的基因工程菌及其构建方法与应用,2022115873221.
联系方式
办公地点:天津经济技术开发区第十三大街9号太阳成集团tyc7111cc
邮政编码:300457
办公电话:022-60912419
传 真:022-60602298
Email:wuheyun@tust.edu.cn